Coding

Part:BBa_M36660:Design

Designed by: Taylor Marie Chavez, Maria Iglesias, Alison Jahansouz   Group: Stanford BIOE44 - S11   (2015-10-23)

mRNA Sequence for Poly-beta-Hydroxybutyrate Polymerase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part was designed to meet the following conditions: be less than 1500 bp, avoid BbsI, BsaI, and BsmBI, and have less than five repeats 10 bp. There was an active attempt to avoid the restriction sites, stated above, that DNA 2.0 uses in the synthesis of DNA and to minimize repeats in the sequence to ensure success during synthesis. A limit of 1500 bp was imposed by the course.


Source

Part comes from the amino acid sequence of Xanthomonas campestris pv. raphani 756C, specifically G0CFI5.

Referenced http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en, which contributed to the discovery of the gene sequence for the necessary protein. DNA sequence was generated from IDT Codon Optimization in E. coli K12.

References

Referenced http://www.uniprot.org/uniprot/G0CFI5 and http://www.google.com/patents/WO1995020621A1?cl=en, which both contributed to the discovery of the gene sequence for the necessary protein.